Tracer tests with fluorescent dyes with respect to those tracing experiments where arrival of dye to water supply systems is expected and Tracer test in Chýnov Karst

The thesis is dedicated to fluorescein tracer tests, especially those where the tracer ends in drinking water. The review part of the thesis describes the usage and characteristics of sodium fluorescein (uranine) and other fluorescent tracers in tracing tests. Special attention is given to the toxicity of fluorescent tracers and to the legislation limiting their use in the Czechia and abroad. The practical part of the work is to perform a fluorescein tracer test from Chýnov Cave to the Rutice Spring on distance 1260 m away. The tracer test has confirmed that the Rutice Spring is the only karst spring where water flows from Chýnov Cave. The results differ from previous tracer tests made on the same flowpath in 1962, 1965 and 1966. While previous studies have concluded that the mean tracer transit time is in the order of tens to hundreds of hours, this tracer test has shown that the mean tracer transit time is in the order of hundreds of days. This work has thus provided completely new knowledge about water flow in the Chýnov karst