Improved Expression of SARS-CoV-2 Spike RBD Using the Insect Cell-Baculovirus System
- Poodts, Joaquín
- Smith, Ignacio
- Birenbaum, Joaquín Manuel
- Rodriguez, María Sol
- Montero, Luciano
- Wolman, Federico Javier
- Marfía, Juan Ignacio
- Valdez, Silvina Noemi
- Alonso, Leonardo Gabriel
- Targovnik, Alexandra Marisa
- Miranda, Maria Victoria
DOIAbstract
Insect cell-baculovirus expression vector system is one of the most established platforms to produce biological products, and it plays a fundamental role in the context of COVID-19 emergency, providing recombinant proteins for treatment, diagnosis, and prevention. SARS-CoV-2 infection is mediated by the interaction of the spike glycoprotein trimer via its receptor-binding domain (RBD) with the host’s cellular receptor. As RBD is required for many applications, in the context of pandemic it is important to meet the challenge of producing a high amount of recombinant RBD (rRBD). For this reason, in the present study, we developed a process based on Sf9 insect cells to improve rRBD yield. rRBD was recovered from the supernatant of infected cel...
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