The specific inhibition of the cardiac electrogenic sodium/bicarbonate cotransporter leads to cardiac hypertrophy
- Di Mattia, Romina Alejandra
- Diaz Zegarra, Leandro Agustin
- Blanco, Paula Graciela
- Valverde, Carlos Alfredo
- Gonano, Luis Alberto
- Jaquenod de Giusti, Carolina
- Portiansky, Enrique Leo
- Vila Petroff, Martin Gerarde
- Aiello, Ernesto Alejandro
- Orlowski, Alejandro
DOIAbstract
Two alkalinizing mechanisms coexist in cardiac myocytes to maintain intracellular pH: sodium/bicarbonate cotransporter (electroneutral isoform NBCn1 and electrogenic isoform NBCe1) and sodium/proton exchanger (NHE1). Dysfunction of these transporters has previously been reported to be responsible for the development of cardiovascular diseases. The aim of this study was to evaluate the contribution of the downregulation of the NBCe1 to the development of cardiac hypertrophy. To specifically reduce NBCe1 expression, we cloned shRNA into a cardiotropic adeno-associated vector (AAV9-shNBCe1). After 28 days of being injected with AAV9-shNBCe1, the expression and the activity of NBCe1 in the rat heart were reduced. Strikingly, downregulation of N...
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