Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
- Scannapieco, Alejandra Carla
- Muntaabski, Irina
- Liendo, María Clara
- Niz, José María
- Russo, Romina Maria
- Salvador, Ricardo
DOIAbstract
The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results eviden...
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