Add to ORKGLoop-mediated isothermal amplification (LAMP) tests have been developedas molecular tests for neglected parasitic diseases such as leishmaniasisor sleeping sickness. A LAMP test for Trypanosoma cruzi, the etiologicalagent of Chagas Disease (ChD), would allow a rapid and reliable diagnosis,in particular in cases of acute and congenital ChD (CChD). We evaluatedthe performance a Trypanosoma cruzi LAMP kit using purified DNA, spikedblood and clinical specimens. Quantitative PCR (qPCR) was used as areference. Different extraction methods for LAMP were also evaluated. TheLAMP reaction was performed at 62.5°C for 45 min. Analytical sensitivitywas measured in ten-fold dilutions of CL Brener (TcVI) and Silvio X10(TcI) DNA. Analytical specificity was...
International audienceBACKGROUND: A century after its discovery, Chagas disease still represents a m...
Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infe...
Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infe...
Early diagnosis of congenital Trypanosoma cruzi transmission in newborns is essential because babies...
A loop-mediated isothermal amplification assay was evaluated as a surrogate marker of treatment fail...
Vertical transmission of Trypanosoma cruzi is the cause of congenital Chagas disease, a re-emerging ...
A Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring...
Chagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic through...
In Spain, PCR is the tool of choice for the diagnosis of congenital Chagas disease (CD) and serology...
In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection...
In Spain, PCR is the tool of choice for the diagnosis of congenital Chagas disease (CD) and serology...
Chagas’ disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic through...
Human African trypanosomiasis (HAT) is one of the neglected tropical diseases in sub-Saharan Africa....
One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector- born...
Human African trypanosomiasis (HAT) is one of the neglected tropical diseases in sub-Saharan Africa....
International audienceBACKGROUND: A century after its discovery, Chagas disease still represents a m...
Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infe...
Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infe...
Early diagnosis of congenital Trypanosoma cruzi transmission in newborns is essential because babies...
A loop-mediated isothermal amplification assay was evaluated as a surrogate marker of treatment fail...
Vertical transmission of Trypanosoma cruzi is the cause of congenital Chagas disease, a re-emerging ...
A Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring...
Chagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic through...
In Spain, PCR is the tool of choice for the diagnosis of congenital Chagas disease (CD) and serology...
In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection...
In Spain, PCR is the tool of choice for the diagnosis of congenital Chagas disease (CD) and serology...
Chagas’ disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic through...
Human African trypanosomiasis (HAT) is one of the neglected tropical diseases in sub-Saharan Africa....
One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector- born...
Human African trypanosomiasis (HAT) is one of the neglected tropical diseases in sub-Saharan Africa....
International audienceBACKGROUND: A century after its discovery, Chagas disease still represents a m...
Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infe...
Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infe...