R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants
- Gonzalo, Lucía
- Tossolini, Ileana del Rosario
- Gulanicz, Tomasz
- Cambiagno, Damián Alejandro
- Kasprowicz Maluski, Anna
- Smolinski, Dariusz Jan
- Mammarella, María Florencia
- Ariel, Federico Damian
- Marquardt, Sebastian
- Szweykowska Kulinska, Zofia
- Jarmolowski, Artur
- Manavella, Pablo Andrés
DOIAbstract
In most organisms, the maturation of nascent RNAs is coupled to transcription. Unlike in animals, the RNA polymerase II (RNAPII) transcribes microRNA genes (MIRNAs) as long and structurally variable pri-miRNAs in plants. Current evidence suggests that the miRNA biogenesis complex assembly initiates early during the transcription of pri-miRNAs in plants. However, it is unknown whether miRNA processing occurs co-transcriptionally. Here, we used native elongating transcript sequencing data and imaging techniques to demonstrate that plant miRNA biogenesis occurs coupled to transcription. We found that the entire biogenesis occurs co-transcriptionally for pri-miRNAs processed from the loop of the hairpin but requires a second nucleoplasmic step ...
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