CBMs in trans: generating alternatives to improve the catalytic efficiency of enzymes

Protein engineering by the addition of substrate binding domains is becoming a widely used strategy to improve enzyme properties. These binding domains are often used to increase the catalytic efficiency, as affinity tags to facilitate protein purification and also for targeting a protein to specific cellular locations. CBMs (carbohydrate binding modules) are non-catalytic protein domains that are naturally present in some enzymes and are associated with the ability to bind polysaccharides.Among CBMs, there is a subgroup called SBDs that have an evolutionary advantage due to the presence of two starch binding sites. The mechanisms of action of CBMs differ and are characteristic of the enzyme in which they are present. They can act by bringing the catalytic domain closer to the substrates, as a scaffold for protein-protein interaction and, additionally, they can break the structure of substrates increasing the catalytic efficiency of the enzyme. The latter is particularly important when the substrates are structured, such as starch granules or plant cell walls.Typically, the fusion of CBMs in cis to generate chimeric proteins is used to evaluate constructs as possible biotechnological tools. Our laboratory has demonstrated the interaction of SBDs in cis with the glycogen synthase (GS) from Agrobacterium tumefaciens. The addition of the D3 domain from Arabidopsis thaliana starch synthase III (SSIII) to the GS conferred a higher capacity for glycogen biosynthesis, suggesting that the careful design of fusion proteins can led to the production of a fully active and conformationally stable molecule composed of domains that belong to different kingdoms,in this case, plants and bacteria. The presence of a polysaccharide-binding site outside the active site of the enzyme would lead to improve the binding capacity through multiple contacts, increasing the local concentration of non-reducing ends in the active site and resulting in a greater processivity of the enzyme.While there are many studies on the effect of CBMs in cis, few studies have been conducted to evaluate their effect in trans. We analysed here the ability of different CBMs, coming from different families (a CBM20 from a Ostreococcus tauri protein (CBM20CP) and three xylan binding domains (XYL1-3, classified in the CBM22 family) from a xylanase from A. thaliana (AtXyn1) to act in trans on two commercial enzymes, an amylase (AmyC) and a xylanase (XYNA) respectively. The CBM20 is located in the central position of a protein without associated catalytic activity. Therefore, we evaluated the effect of the recombinant CBM20 and the full CBM20CP. We found that the addition of CBM20 had a little impact on the activity of AmyC, while the addition of the full protein significantly increased (about 90%) the catalytic efficiency of the enzyme. On the other hand, of the three CBMsfrom AtXyn1 used, only XYL1 was able to increase the Vmax of XYNA by 70% without significant differences in the Km, suggesting that the increase in the catalytic efficiency is exclusively due to the increase in Vmax and not in its affinity for the substrate. Our results show that the addition in trans of CBM20CP and XYL1 would be a useful strategy to improve the activity of some enzymes that use different polysaccharides as substrates.Fil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Grisolía, Mauricio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Hedin, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaTercer Encuentro de Red Argentina de Tecnología Enzimática; Primer Workshop de la Red Argentina de Tecnología EnzimáticaRosarioArgentinaRed Argentina de Tecnología Enzimátic