Comparison of dairy phospholipid preparative extraction protocols in combination with analysis by high performance liquid chromatography coupled to a charged aerosol detector

Barry, Kate M.
Dinan, Ted G.
Murray, Brian A.
Kelly, Philip M.

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Publication date

May 2016

DOI

10.1016/j.idairyj.2016.01.022.

Publisher

Elsevier

Abstract

peer-reviewedDifferent fat extraction methodologies, Röse Gottlieb (RG), Folch with CaCl2 (F1) and Folch without CaCl2 (F2) were evaluated alongside a high performance liquid chromatography (HPLC)-based analytical protocol involving a charged aerosol detector (CAD) to optimise the separation and quantification of dairy phospholipids (PLs). Total PL recovery by F2 proved to be 1.8- and 2.5-fold greater than RG and F1, respectively. Alteration of the HPLC elution program maximised peak separation and reduced co-elution of individual PLs detected by CAD. Two-fold reduction in retention time variability was achieved, <0.15 min, compared with the original method. Improved PL recovery was reflected in the higher PL values obtained from different ...

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Comparison of dairy phospholipid preparative extraction protocols in combination with analysis by high performance liquid chromatography coupled to a charged aerosol detector

Abstract

Extracted data

Comparison of dairy phospholipid preparative extraction protocols in combination with analysis by high performance liquid chromatography coupled to a charged aerosol detector

Abstract

Extracted data

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