Proteomic results and Gene Ontology analysis for five wild-type femurs.

Proteins identified in wild-type mouse femurs were analyzed using ConsensusPathDB-mouse (Release MM11, 14.10.2021) GO for both A) “cellular compartment” and B) “biological processes.” The most enriched cellular compartments were the cytoplasm and nucleus while the most significant GO Biological Process was “collagen fibril organization”. C) Protein sequence coverage for secreted protein acidic and cysteine rich (SPARC), an osteoblast marker, is highlighted where green sections of the sequence (51%) are confidently identified in the proteomics data. D) directDIA generated Pseudo-MS/MS spectrum (constructed from DIA generated spectral library) and extracted ion chromatogram of the SPARC peptide LEAGDHPVELLAR (m/z = 710.38, z = 2+) illustrates peak quality and intensity for 2 selected samples, WT1 and WT3.