CD3 staining and quantification.

Representative images of immunohistochemistry staining of CD3 in lungs of A. C57BL/6 mice. Images are from the three treatment cohorts (control, isoflurane, and ketamine) and contain representative high-power fields with inset low-power images of the entire tumor cross-section. Positivity mask in lower panels. Percent positive cells quantified over an entire viable area of lung cross-section of B. C57BL/6. Mean ± SEM, n = 3 mice in each arm. One-way ANOVA, (F2,6 = 14.86, p = 0.0047). **p < 0.0029 and *p < 0.0495 vs. control group.