Time course of WD effects hepatic ω6 PUFA-derived oxylipins.

A and B: The heat map represents the average abundance of non-esterified ω6-fatty acids (18:2, ω6 and 20:4, ω6) and oxylipins derived from these fatty acids normalized to hepatic protein, i.e., Peak area/mg hepatic protein. 18:2,ω6, LA, linoleic acid; 9(S)-HODE, 9S-hydroxy-10E,12Z-octadecadienoic acid; 13(S)-HODE, 13S-hydroxy-9Z,11E-octadecadienoic acid; 9,10-EpOME, (±)9,10-epoxy-12Z-octadecenoic acid; 12,13-EpOME, 12(13)epoxy-9Z-octadecenoic acid; 9,10-DiHOME, 9,10-dihydroxy-12Z-octadecenoic acid; 12,13-DiHOME, 12,13-dihydroxy-9Z,octadecenoic acid; 20:4,ω6, ARA, arachidonic acid; PGD2, prostaglandin D2; PGE2, prostaglandin E2; 15-Keto PGE2, 15 keto prostaglandin E2; PGJ2, prostaglandin J2; 6-keto PGF1α, 6-keto prostaglandin F1α-d4; 8-iso PGF2α, 8-iso prostaglandin F2α; 11 β-PGF2α: 11-Epi-prostaglandin F2α; 15-keto PGF2α, 15-keto-prostaglandin F2α; 15-deoxy-Δ12,14-PGJ2, 15-Deoxy-Δ12,14-prostaglandin J2; Leukotriene B4; Thromboxane B2; 5(S)-HETE: 5-Hydroxyeicosatetraenoic acid; 8(S)-HETE: 8-hydroxyeicosa-tetraenoic acid; 9(R)-HETE: 9R-hydroxy-5Z,7E,11Z,14Z-eicosatetraenoic acid; 11(S)-HETE, 11S-hydroxy-5Z,8Z,12E,14Z-eicosatetraenoic acid; 12(S)-HETE: 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid; 15(S)-HETE: 15-Hydroxyeicosatetraenoic acid; 20-HETE: 20-Hydroxyeicosatetraenoic acid; 11,12-EET: 11,12-Epoxyeicosatrienoic acid; 14,15-EET: 14,15-Epoxy-5Z,8Z,11Z-eicosatrienoic acid; 11,12-DiHET: 11,12-Dihydroxy-5Z,8Z,14Z-eicosatrienoic acid; 14,15-DiHET: 14,15-Dihydroxy-5Z,8Z,11Z-eicosatrienoic acid. Statistical differences (p C–D: Graphs for LA- and ARA-derived oxylipins: C. 9(S)-HODE, D. PGE2 and E. 5(SHETE) are presented as peak area/mg hepatic protein. Statistical significance (p < 0.05) at 1 and 40 wks for WD versus LFD is designated by an asterisk (ӿ).