Proteomic and metabolomic profiling of AAC DKO cells.

(A) Volcano plots showing a comparison of protein expression levels (3654 protein groups) between BSF 427 and AAC DKO cells. Log2 fold change values of averaged LFQ intensities from quadruplicate experiments are plotted against the respective −log10-transformed P values. Significantly changed hypothetical proteins are shown in blue, down-regulated cytosolic ribosomal proteins are shown in dark red. Mitochondrial enzymes involved in amino and keto acid oxidation including TCA cycle enzymes are highlighted in orange. ME, malic enzyme; IDH, isocitrate dehydrogenase; SDH1, succinate dehydrogenase subunit 1; AAT, alanine aminotransferase; PDH E3, subunit of pyruvate dehydrogenase; KDH E2, subunit of α-ketoglutarate dehydrogenase; BCKD E2, subunit of branch chain keto acid dehydrogenase; MPC2, mitochondrial pyruvate carrier 2; MCP14, mitochondrial carrier protein 14. (B) Volcano plot showing the detected metabolites (124 metabolites) analyzed in BSF 427 and AAC DKO cells. Log2 fold change values of the average of mean peak area from quadruplicate experiments are plotted against the respective −log10 transformed P values. AAs, amino acids. (TIF)