Co-culture growth of <i>Lp. plantarum</i> and <i>A. pasteurianus</i>.

Lp. plantarum and A. pasteurianus were grown in co-culture in CDMA with glucose and potassium acetate added. (A) Growth curves (solid lines) display means of 12 experimental replicates. Shaded areas represent standard error on a 5-point rolling average. Time point OD600 readings were taken every 5 minutes. (B) OD measurements were taken at the end of the growth curve after resuspension of the culture supernatant for each of the 12 replicates. Mean and standard deviation are shown. We note that the apparently 4-fold lower OD of Lp. plantarum in the resupsended culture versus the time course in A is likely due to settling of the cells and adherence to the plate during the time course. The CFU/mL counts are in C. (C) CFU/ml was counted by plating the resuspended cultures on MRS and MYPL for each of the 12 replicates. Mean and standard deviation shown.