Nβ motif is mostly located towards the N-terminus of extracellular proteins.

(A) Distribution of the proteins according to the localisation of the Nβ motif. The sequences were divided into four quarters: P1: 25% of the protein towards the N-terminus; P2 and P3: second and third quarters, respectively; P4: 25% of the protein towards the C-terminus. (B) Annotated functions of the proteins that contain the Nβ motif in each position. PI: peptidases and inhibitors; RibB: ribosomal biogenesis; Chap: chaperones; Aut: autophagy; ER: endoplasmic reticulum; MR: membrane recycling; TF: transcription factors; LM: lipid metabolism; End: endocytosis; Ub: ubiquitination; Exo: exocytosis; Lys: lysosome; MT: membrane transport; Exo(synth): exocytosis (synthesis); DR: DNA repair; RB: RNA biogenesis; SO: sarcomere organization; Unk: unkown. (C) Distribution of the cellular localisation based on the categories generated using the UniProtKB database in each position (colour code is the same as in Fig 3A). P1, P2, P3 and P4 labels between (B) and (C) refer to upper (B) and lower (C) pie-charts.