Colistin MICs of transposon and engineered mutants. A.

MIC50 of mutants with transposon insertions. The transposon insertion sites are shown. B. MIC50 of mutants with mutations engineered in single genes and of evolved mutants with single mutations in pmrB. C. MIC50 of multi-gene mutants. Biological replicates are displayed as individual data points, with black lines indicating the median values.