Short communication: The relationship between dietary particle size and undegraded neutral detergent fibre in lactating dairy cows: A prospective cohort observational study

Abstract Physically effective NDF (peNDF) and undegraded aNDF at 240 h (uNDF.240) are important parameters for characterizing NDF in fibre evaluation and are associated with dietary physical form and fibre digestibility characteristics. A new concept that combines peNDF and uNDF.240, physically effective uNDF.240 (peuNDF.240 = pef × uNDF.240), was recently established. The peuNDF.240 value allows determination of dry matter intake (DMI), and the productive response of cows even in the absence of variation in DMI or when cows are fed rations with low uNDF.240 and high peNDF or rations with high uNDF.240 and more finely chopped fibre. The aim of this study was to improve our understanding of the relationships between dietary uNDF.240 content to other fibre fractions, average cow DMI, gross feed efficiency, and milk yield at the farm level. Furthermore, the relation between peuNDF.240 and the productive response of cows was also investigated at the farm level. In the Po' Valley, which is a representative area for dairy production in Italy, a cohort of 22 Holstein dairy farms was monitored over two years (2019–2020). Information regarding average cow DMI, milk yield, and ration composition was obtained through interviews with farmers, and feed samples were collected and chemically analysed. Farms were classified according to their dietary uNDF.240 (% of DM) content: low (uL) ≤ 8.29 or high (uH) > 8.29. Farms with low dietary uNDF.240 used less alfalfa forage as a fibre source compared with farms with high dietary uNDF.240 (6.27 vs. 15.5 % of DM) and showed higher average milk yield (35.9 vs. 33.6 kg/cow/day, respectively) and similar DMI (23.9 vs. 24.3 kg/cow/day, respectively). Dietary peuNDF.240 was negatively related to milk yield (milk yield = 47.4 – 1.87 peuNDF.240, R2 = 0.62, adjusted R2 = 0.60, residual standard error (RSE) = 1.87, P = 0.001) and gross feed efficiency (gross feed efficiency = 1.96 – 0.08 peuNDF.240, R2 = 0.65, adjusted R2 = 0.64, RSE = 0.07, P = 0.001). The results of this study have practical significance for farmers, as they suggest that the inclusion of low digestible forages in the ration (i.e., late-harvested alfalfa characterized by high uNDF.240) may require more fine shredding to reduce the overall value of peuNDF.240 and increase cow production