Novel blocked functionality copolymers as surface coatings in DNA microarray technology

AbstractNew copolymers made of 2-(dimethyl amino) ethyl methacrylate (DMAEM) and isocyanate ethyl methacrylate blocked with methyl ethyl ketoxime (IEMB) was synthesized in various composition ratios in order to obtain water reducible copolymers after acidification. They were characterized by infrared spectroscopy, chemical titration for the determination of amine equivalent weight, and by differential scanning calorimetry (DSC). Calorimetric analysis showed the presence of a former broad endothermal peak followed by a latter, stronger exothermal one, which can be respectively attributed to the deblocking of isocyanate groups and to their subsequent reactions. Some copolymer compositions were dip coated onto microscope glass slides and surfaces were characterized by static and dynamic contact angles, revealing a clear effect of the salifying agent and a likely surface rearrangement of polar groups while in contact with water. Finally, oligonucleotides surface immobilization and molecular recognition capability of the coated glass slides were positively assessed by hybridization tests with fluorescently labelled complementary probes