Peer-reviewed dataset: The quantitative and condition-dependent Escherichia coli proteome

Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities. SpeciesList: scientific name: Escherichia coli; NCBI TaxID: 562; ModificationList: Oxidation; Acetyl; Carbamidomethyl Instrument: LTQ Orbitrap Elite Cell Type: permanent cell line cell Instrument: LTQ Orbitrap Elite Software: Mascot Parser 2.4.3.0, Mascot Server 2.4.1 Modification: Acetyl; Oxidation; Carbamidomethyl Quantification: TIC Experiment Type: Shotgun proteomic