Quantitave Histochemical Study of Mucus Sccreting Cells in Human Maxillary Mucosa

正常成人27例,成人慢性副鼻腔炎患者59例の上顎洞粘膜分泌細胞の定性,定量的検討を行った.1)単位長さあたりの杯細胞数は,上顎洞粘膜では,部位による差を認めなかった.対照群と副鼻腔炎群間で,杯細胞数の変化はみられなかった.2)上顎洞組織中における一定面積あたりの粘膜固有層腺細胞の占有面積の割合は,対照群において,後壁に比し自然孔付近でより大であった.慢性副鼻腔炎群では,後壁,自然孔付近ともに粘膜下腺細胞が増加しており,ことに,後壁でその傾向が著しかった.3)杯細胞中のムコ多糖は,対照群では,後壁,自然孔付近ともに中性ムコ多糖が75%以上を占めた.慢性副鼻腔炎群では,中性ムコ多糖がほとんど消失し,酸性ムコ多糖が90%以上であった.4)粘膜固有層腺細胞中のムコ多糖は,対照群では,後壁,自然孔付近ともに中性ムコ多糖が90%以上であり,杯細胞におけるよりその占有率が高かった.副鼻腔炎群では,シアリダーゼ感受性シアル酸ムコ多糖と硫酸ムコ多糖が中心として存在し,この両者をあわせると,後壁,自然孔付近ともに約75%であったHistochemical quantitation of mucopolysaccharides jn the maxillary mucosa was performed on postmortem specimens. The posterior and medial walls of the maxillary sinuses were removed from 27 adults with normal sinuses and 57 adults with chronic sinusitis. Both goblet cells and submucosal gland cells were stained by Alcian blue at various pH levels and with periodic acid-Schiff with or without sialidase digestion. There was no significant difference in the number of goblet cells between the normal and chronic sinusitis specimens. Submucosal gland cells were more dense in the sinusitis than in the normal tissues, especially in the posterior maxillary wall specimens. In the goblet cells, more than 75% of the mucopolysaccharide was neutral in the normal group, and more than 90% was sulphated in the sinusitis group. In the submucosal layers, 90% of the gland cells contained neutral mucopolysaccharides in the normal group, while sulphated and sialidase sensitive mucopolysaccharides were present in 75% of the cells in the sinusitis group