アカシオ プランクトン Noctiluca milialis ノ ステロール

The sterols of a dinoflagellate, Noctiluca milialis, was investigated. The specimens of N.milialis were collected from the sea water where a red water happened. Sterols were isolated and then separated into six fractions by thin-layer chromatography on 10% AgNO_3-Kieselgel G. The sterol components of six fractions were identified by a combined gas-chromatography and mass spectrometry. N.milialis contained cholesta-5, 22E-dienol (4.8%), 5α-cholestanol (5.6%), 24-methylcholesta-5, 22E-dienol (72.5%), 24-methylenecholesterol (5.9%), and small amounts of other fifteen components. Interestingly, N.milialis contained 4α-monomethyl-sterols such as 4α-methylcholestanol, 4α, 24-dimethylcholest-22-enol, 4α, 24-dimethylcholestanol, 4α-methyl-24-ethylcholest-22-enol, and 4α-methyl-24-ethylcholestanol as minor components (