This study assessed the groundwater quality of 30 selected wells and boreholes in Iwo Local Government Area, Osun State, Nigeria. Groundwater sources were randomly stratified and identified according to the 15 political wards using hand-held GPS equipment. The sources were sampled during the rainy season (October) and dry season (January) to determine water quality. The physico-chemical and microbiological parameters of the water samples such as temperature, turbidity, total suspended solids, pH, electrical conductivity, total dissolved solids, total alkalinity, total hardness, chloride, sulphate, nitrate, phosphate, magnesium, calcium, iron, zinc, lead, manganese, cadmium, chromium, and total coliform were determined using standard methods...