Simultaneous Detection of Chlamydia Trachomatis, Neisseria Gonorrhoeae, Ureaplasma Urealyticum by Multiplex PCR-Running

Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU) and Neisseria gonorrhoeae (NG) are the most common pathogens of sexually transmitted infections (STIs), frequently founded in urogenital infections, and showed a criminal role in increasing the risk of potential adverse outcomes. In this study a multiplex PCR assay for the simultaneous detection and accurate identification of 3 clinically relevant pathogens of STIs, i.e., CT, NG and UU in a single tube was developed and evaluated. The limits of detection for the multiplex PCR assay were ~10 copies of DNAs per reaction. This assay has comparable clinical sensitivity to the conventional monoplex real-time PCR assay and considerable potential to be routine molecular diagnostic tool for simultaneous identification of STIs at relatively low cost due to multiplexing