Comparative transcriptome analysis of the hepatopancreas of Eriocheir sinensis following oral gavage with enrofloxacin

Our study produced 80,228,728 and 88,888,706 raw reads from control (kongbai3) and treatment (shiyan4) groups, and after filtering and quality checks of the raw sequence reads, our analysis yielded 78,843,613 and 87,628,922 clean reads with an average length of 126 bp from control and treatment groups, respectively. A total of 15,797 transcripts were assembled, with 11,975 transcripts were annotated. Moreover, 2,795 transcripts were judged to be differentially expressed genes. GO terms biological process and metabolic process were the most enriched in the oxidation-reduction process, translational initiation, membrane, cytoplasmic part and hydrolase activity. KEGG pathway analysis showed that metabolic and signal transduction pathways were significantly enriched. Furthermore, we found that gshB and the CYP450 enzyme system plays a role in the metabolism of enrofloxacin in the hepatopancreas of E. sinensis. This study identified differential unigenes related to transmembrane transport and drug metabolism in Eriocheir sinensis which could help us developing our understanding of the molecular basis of enrofloxacin metabolism in this economically important aquaculture speciesThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author