EFFECTS OF FLAXSEED AND ITS COMPONENTS ON THE INTESTINAL MICROBIOTA AND MAMMARY GLAND MICRORNA SIGNATURE IN FEMALE C57BL/6 MICE

Flaxseed (FS) is an oilseed rich in α-linolenic acid, lignans, and dietary fiber. Due to this unique composition, FS has potential to affect the health of the gut and breast. This study determined the effect of FS and its components, FS oil (FSO) and secoisolariciresinol diglucoside (SDG), on the gut microbiota, serum concentration of microbial-derived SDG metabolites, and mammary gland microRNA signature. It was found that animals maintained on 10% FS experienced the most prominent changes in gut microbiota composition and diversity compared to other groups. Higher concentrations of SDG metabolites were detected in the 10% FS and SDG diet groups, while not detectable in FSO or control. Lastly, four microRNAs in the mammary gland were downregulated in the experimental diet groups indicating that the mammary gland microRNA signature responded to FS and its components.M.Sc.2020-11-15 00:00:0