Morphometric analysis of structural renal alterations and beneficial effects of aminoguanidine in acute kidney injury induced by cisplatin in rats

Since cisplatin-induced nephrotoxicity has very important clinical consequences, purpose of this study was to determine the potential protective effect of aminoguanidine on the acute kidney injury caused by cisplatin. Experiments were done on 40 Wistar rats divided into four groups. CIS group received cisplatin in a single dose of 8 mg/kg, while CISAG group received the same dose of cisplatin and aminoguanidine (100 mg/kg) by intraperitoneal injections. Animals in AG group received only aminoguanidine (100 mg/kg) and those in C group received saline. Quantitative evaluation of structural and functional alterations in the kidneys were performed by analysis of biochemical and parameters of oxidative stress and by histological and morphometric analysis of renal sections. Histological sections of kidney ameliorated structural damages of proximal tubules and glomeruli which were induced by cisplatin. Morphometric analysis revealed statistically significant differences in the area of proximal tubules, size and cellularity of glomeruli between CIS and CISAG group. Glomerular basement membrane thickness was increased in CIS group, while aminoguanidine attenuated these changes in CISAG group of rats. Our results suggest that aminoguanidine acts protectively and repairs structural and functional damages of kidney by engaging the existent antioxidative potential at the level of renal tissue.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author