Molecular Characterization of Preeclampsia In Vivo and In Vitro

Preeclampsia (PE) is a hypertensive disorder of pregnancy characterized by poor placental development. Unfortunately, variations in PE pathogenesis have made management and development of effective intervention agents difficult. An improved understanding of the molecular basis of PE is required to properly classify this complex disease, and determine effects drugs may have on molecular profiles. Here, we sought to characterize the molecular profiles present in PE, and intervention agent heparin's effect on these profiles. Proteomic analysis of placentas from the BPH5 PE mice model revealed differential protein expression and increased Stat3 phosphorylation compared to wild-type mice. Increased Stat3 phosphorylation was also observed in an additional in vivo, and one in vitro PE model. Microarray and phospho-proteomic analysis revealed differential effects of heparin on molecular profiles in in vivo and in vitro PE models. Identifying molecular pathways altered and which pathways heparin targets in PE may aid in designing individualized therapeutic approaches.M.Sc.2017-02-28 00:00:0