Differential expression of six obesity-related genes with different disease phases of T2DM in cynomolgus monkey

Type 2 diabetes mellitus (T2DM) is a metabolic disease with a strong genetic component that is very prevalent in the world. The aim of this study is to investigate the association of a set of six obesity-related genes with the different disease phases of T2DMin a model using middle or aged cynomogus monkeys. A total of 25 male monkeys were used and fed with high-fat diet (15% lard). The disease development and progression of T2DM were monitored through the levels of plasma glucose and lipid. The mRNA expression of 6 genes was evaluated using real-time PCR on monocyte isolated from monkey peripheral blood. The 2-hour plasma glucose levels followed oral glucose tolerance test (OGTT) were (11.06±6.05) mmol/L and (13.12±2.89) mmol/L respectively (P<0.01), and the fasting plasma glucose level was (7.58±1.56) mmol/L (vs controls, P