We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotry...
The proteolytic activity of High-Five insect cell culture supernatants was analysed using substrate ...
Author Institution: Department of Zoology and Entomology, The Ohio State University, Columbu
Samples were prepared from the midgut of 4th instar larvae of the crimson speckled moth Utethesia pu...
We report for the first time the expression of multiple protease activities in the first instar larv...
We report for the first time the expression of multiple protease activities in the first instar larv...
Serine proteases are the major proteolytic activity excreted or secreted from Chrysomya bezziana lar...
Sarconesiopsis magellanica (Diptera: Calliphoridae) is a medically important necrophagous fly which ...
The diversity of serine proteases secreted from Chrysomya bezziana larvae was investigated biochemic...
Proteolytic activity in excretory/secretory products (ESP) of first- (L1), second- (L2) and third-in...
AbstractWe have reported a novel serine protease produced by Sarcophaga peregrina (Nakajima et al., ...
The oriental fruit fly Bactrocera dorsalis (Hendel) causes devastating losses to agricultural crops ...
Digestive proteolytic profile was determined in the larvae of <em>Dyspessa palidata</em> (Staudinger...
This work describes the purification and characterization of a trypsin-like enzyme with fibrinolytic...
Protease inhibitors mediate a natural form of plant defence against insects, by interfering with the...
A novel chymotrypsin which is expressed in the midgut of the lepidopteran insect Spodoptera exigua i...
The proteolytic activity of High-Five insect cell culture supernatants was analysed using substrate ...
Author Institution: Department of Zoology and Entomology, The Ohio State University, Columbu
Samples were prepared from the midgut of 4th instar larvae of the crimson speckled moth Utethesia pu...
We report for the first time the expression of multiple protease activities in the first instar larv...
We report for the first time the expression of multiple protease activities in the first instar larv...
Serine proteases are the major proteolytic activity excreted or secreted from Chrysomya bezziana lar...
Sarconesiopsis magellanica (Diptera: Calliphoridae) is a medically important necrophagous fly which ...
The diversity of serine proteases secreted from Chrysomya bezziana larvae was investigated biochemic...
Proteolytic activity in excretory/secretory products (ESP) of first- (L1), second- (L2) and third-in...
AbstractWe have reported a novel serine protease produced by Sarcophaga peregrina (Nakajima et al., ...
The oriental fruit fly Bactrocera dorsalis (Hendel) causes devastating losses to agricultural crops ...
Digestive proteolytic profile was determined in the larvae of <em>Dyspessa palidata</em> (Staudinger...
This work describes the purification and characterization of a trypsin-like enzyme with fibrinolytic...
Protease inhibitors mediate a natural form of plant defence against insects, by interfering with the...
A novel chymotrypsin which is expressed in the midgut of the lepidopteran insect Spodoptera exigua i...
The proteolytic activity of High-Five insect cell culture supernatants was analysed using substrate ...
Author Institution: Department of Zoology and Entomology, The Ohio State University, Columbu
Samples were prepared from the midgut of 4th instar larvae of the crimson speckled moth Utethesia pu...