Collective Epithelial Cell Migration in vitro Driven by Mechanical and Chemical Cues

Cells in vivo respond to chemical and mechanical cues in the environment. In fact, it is the resulting migration of cells as a cohesive group that underlies embryonic morphogenesis, wound repair and cancer tumour development and invasion. Techniques have been developed to investigate chemotaxis, haptotaxis and mechanotaxis – the directional movement of cells in response to soluble chemical cues, substrate-bound chemical cues and mechanical cues respectively. Most of the existing tools however, have been designed for and applied to the investigation of single cell migration. Given its importance in vivo, there is a need for adapting these methods and applying them to characterize directed collective cell migration. The main objective of my thesis was to engineer tools and quantitative methods to investigate collective cell migration and use them to compare single and collective migration in response to mechanical cues and substrate-adhered chemical cues in vitro.MAS