Growth of Leukemic Cells in Culture

This research was originally published in Blood. AYE, M. T., TILL, J. E., & MCCULLOC.EA. (1972). Growth of leukemic cells in culture. Blood-the Journal of Hematology, 40(6), 806-811. © the American Society of Hematology.Peripheral blood cells from three patients with leukemia in relapse increased in numbers in short-term suspension cultures. This increase was dependent on the presence of either feeder populations containing a high percentage of blast cells or conditioned medium derived from such populations. Aneuploid cells present in direct marrow preparations were also prevalent in the cultures after 10 days. The apparent specificity of the increase for leukemic populations may provide a new approach to the study of leukemic blast cells.Supported by Grant MT-1420 from the Medical Research Council, Canada, and Grant 235 from the Ontario Cancer Treatment and Research Foundation