Biodistribution study of the anaesthetic sodium phenobarbital labelled with Technetium-99m in Swiss mice Infected with Schistosoma mansoni Sambon, 1907

Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 92(5), September/October 1997, pp. 677-681 Biodistribution study of the anaesthetic sodium phenobarbital labelled with Technetium-99m in Swiss mice Infected with Schistosoma mansoni Sambon, 1907 Susana Balmant Emerique Simoes, Jose Roberto Machado-Silva, Bianca Gutfilen, Octavio Augusto Franca Presgrave, Marcia BetAnia Oliveira, Mario Bernardo-Filho Code Number:OC97127 Sizes of Files: Text: 17.2K Graphics: Tables (jpg) - 143.9K Technetium-99m (^99mTc) is a radionuclide that has negligible enviromnental impact, is easily available, inexpensive and can be used as a radioactive tracer in biological experiences. In order to know the mode of action of sodium phenobarbital in moving adult Schistosoma mansoni worms from mesenteric veins to the liver, we labelled sodium phenobarbital (PBBT) with ^99mTc and a biodistribution study in infected and non-infected Swiss mice was performed. The PBBT was incubated with stannous chloride used as reducing agent and with ^99mTc, as sodium pertechnetate. The radioactivity labelling (%) was determined by paper ascending chromatography perfomed with acetone (solvent). The ^99mTc-PBBT was administered by intraperitoneal route to Swiss mice infected eight weeks before. The animals were perfused after diferent periods of time (0,1,2,3,4 hr) when blood, spleen, liver, portal vein, mesenteric veins, stomach, kidneys and adult worms were isolated. The radioactivity present in these samples was counted in a well counter and the percentage was determined. The radioactivity was mainly taken up by the blood, kidney, liver and spleen. No radioactivity was found on the adult worms. We concluded that the worm shift was due to an action on the host of the sodium phenobarbital. Key words: Schistosoma mansoni - technetium-99m-sodium phenobarbital - biodistribution - Swiss mice Copyright 1997 Fundacao Oswaldo Cru