Anti-VP1 and Anti-VP2 Antibodies Detected by Immunofluorescence Assays in Patients with Acute Human Parvovirus B19 Infection

Mem Inst Oswaldo Cruz 2001, Volume 96, Number 4, pp. 507-513 Anti-VP1 and Anti-VP2 Antibodies Detected by Immunofluorescence Assays in Patients with Acute Human Parvovirus B19 Infection Renata FA Pereira, Walquiria NS de Paula, Rita de Cássia N Cubel, Jussara P Nascimento Code Number: oc01077 ABSTRACT: Acute human parvovirus B19 infection is followed by an antibody response to the structural proteins of the viral capsid (VP1 and VP2). We used 80 sera collected from 58 erythema infectiosum and 6 transient aplastic crisis patients to test IgM and IgG antibodies against these two proteins in an immunofluorescence assay (IFA) using Sf9 cells infected with recombinant baculovirus expressing either VP1 or VP2 antigen. Although less sensitive than IgM capture enzyme immunoassay using native antigen (MACEIA), we could detect anti-VP1 or anti-VP2 IgM antibodies by IFA in 49 patients with acute infection (76.6%). Detection of IgG anti-VP1 and anti-VP2 by IFA, however, was as sensitive as IgG detection by indirect enzyme immunoassay. By applying IgG avidity IFA to sera of the 15 IgM IFA negative patients we were able to confirm acute infection in further 12 cases by IFA. Overall, acute infection was confirmed by IFA in 61 (95.3%) of the 64 patients. KEYWORDS: human parvovirus B19, VP1 and VP2 structural proteins, IgG and IgM antibodies - immunofluorescence, IgG avidity Copyright 2001 Mem Inst Oswaldo Cru