Continuous ethanol production by Escherichia Coli KO11 in continuous stirred tank and fluidized bed fermenters

Australasian Biotechnology, Volume 7 Number 4, September/October 1997, pp.300-303 Continuous ethanol production by Escherichia Coli KO11 in continuous stirred tank and fluidized bed fermenters Dumsday, G.J., Zhou, B., Buhmann, S., Stanley, G.A. and Pamment, N.B. Code Number: AU97039 Size of Files: Text: 13.7K Graphics: Line drawings (gif) - 21.7K Escherichia coli KO11 was grown for 16 and 27 days respectively in a continuous stirred tank reactor with cell recycle (CSTR) and a fluidized bed reactor. The substrate was 50 g/l glucose. Average ethanol yields of 0.44 g ethanol/g sugar added were achieved in the CSTR and 0.40 g ethanol/g sugar added in the fluidized bed. The ethanol productivities were 1.27 gl^- 1 h^-1 and 1.12 gl^-1 h^-1 respectively at a dilution rate of 0.06 h^-1. By-product concentrations were comparatively low and there was no time-dependent loss in ethanologenicity. The results justify further testing of E. coli KO11 in continuous fermentation systems. Copyright 1997 Australian Biotechnology Association Ltd