Nucleotide sequence and recombinant expression in Escherichia coli of coat protein gene from a cuban isolate of TYLCV

Biotechnologia Aplicada 1996; Vol 13, No.2 Orlene Guerra Pedro Ramos Lisset Herrera Vivian Dorestes Maria Lopez Nadia Ramirez and Pedro Oramas Code Number: BA96050 Sizes of Files: Text: 3.8K Graphics: Line drawings (gif) - 2.5K Photographs (jpg) - 34.1K Introduction Typical symptoms of TYLCV (tomato yellow leaf curl virus) infections in tomato plants have been observed in Cuba since 1987 (1). TYLCV, a whitefly transmitted geminivirus, became the most devastating disease of tomato in Cuba, causing up to 100 % crop loss. Here, we report the nucleotide sequence of coat protein (cp) gene and its expression in E. coli in order to characterize the TYLCV Cuban isolate (TYLCV-Cu) and obtain antiserum against it. Copyright 1996 Elfos Scientia