RAPD Analysis on Guizhou Native Goat Breeds

We used 180 primers in RAPD analysis of Guizhou native goats (the small-xiang goat,Guizhou white goat,Guizhou black goat and Qianbei-pockmarked goat),Nanjiang brown goats and Boer goats.Of the primers,27 amplified polymorphic patterns,including 281 bands.Of the bands,115 were polymorphic and their frequency was 40.92% on average (range 20%-80%).Each of the 27 primers had 10.41 bands on average (range 4-16),and the fragment length of products was 210-2 800 bp.Genetic distance index appeared the lowest between the Guizhou black goat and Guizhou white goat (0.0605),and the highest between the Boer goat and the other goats (0.1059-0.1488).NJ tree indicated that the Guizhou white goat was closely related to the Guizhou black goat,and the next was the Qianbei-pockmarked goat;the small-xiang goat was farther from the other Guizhou native goats than Nanjiang brown goat from.The results analysis suggest that the small-xiang goat is a independent breed on genetics,and Guizhou native goat breeds have the close genetic relationship,low genetic variability and high genetic stability