Optimization of in vitro regeneration of multiple shoots from hypocotyl sections of cotton (Gossypium hirsutum   L.)

A direct shoot regeneration protocol was optimized for cotton (Gossypium hirsutum ). Two cultivars of cotton, Delta pine 50 (DP50) and Stoneville 474 (STV474) were used to study the effects of thidiazuron (TDZ), naphthalene acetic acid and silver nitrate (AgNO3) on shoot regeneration from hypocotyl explants excised from 14 day-old seedlings cultured in vitro. The best treatment for formation of adventitious shoots in DP50 was the treatment containing 0.175 mg l-1 TDZ, 0.01 mg l-1 NAA and 5.1 mg l-1 AgNO3, while treatment containing 0.08 mg l-1 8 TDZ, 0.01 NA mg l-1 A and mg l-1 10.2 AgNO3 was optimum for the formation of adventitious shoots for STV474