Nutritional recovery from low protein diet during pregnancy does not restore the kinetics of insulin secretion and Ca2+ or alterations in the cAMP/PKA and PLC/PKC pathways in islets from adult rats

We investigated the insulin release induced by glucose, the Ca2+ oscillatory pattern and the cAMP/PKA and PLC/PKC pathways in islets from adult rats that were reared under diets with 17% protein (C) or 6% protein (LP) during gestation, suckling and after weaning and in rats receiving diets with 6% protein during gestation, followed by a 17% protein diet after birth (R). Glucose-induced first-phase insulin secretion was reduced in LP and R islets, and the second phase was partially restored in the R group. Glucose stimulation did not modify [Ca2+]i, but it reduced the [Ca2+] oscillatory frequency in the R group compared to the C group. [cAMP]i was higher, and PKAÎą expression was lower in the R and LP groups compared to the C group. The PKCÎą content in islets from R rats was lower than in C and LP rats. Thus, nutritional recovery from low protein during fetal life did not repair the kinetics of insulin release, impaired the Ca2+ handling and alterated the cAMP/PKA and PLC/PKC pathways.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author