Comparing compound-specific and bulk stable nitrogen isotope trophic discrimination factors across multiple freshwater fish species and diets

Compound-specific nitrogen stable isotope analysis provides an approach for estimating animal trophic position that may overcome key issues associated with stable isotope analysis of bulk tissue. Yet compound-specific trophic discrimination factors have not been estimated for a broad range of habitats, taxa, and diets. We conducted a controlled-feeding experiment to characterize the variation in compound-specific (TDFAA) and bulk (TDFBulk) trophic discrimination factors of four freshwater fish species fed on three distinct diets. We also compared TDFAA of fish muscle and scale to evaluate the viability of scales for making food web inferences. Mean TDFBulk was 2.2±0.9‰ (±1 SD) and there were significant effects of species and diet trophic position on TDFBulk. Mean TDFAA was 6.9±0.8‰ (±1 SD). Although there was no effect of species on TDFAA, there were significant differences in TDFAA across the three diets. TDFAA from fish scales were not significantly different from those of muscle. Our study illustrates the advantages of estimating trophic position using compound-specific stable isotopes and the need for continued investigation of factors resulting in variation in TDF values.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author