Temperature‐sensitive (TS) alleles create tunable thermoswitches to deplete essential cellular activities and are used to dissect gene function. In their recent study, Link and colleagues (Schramm et al 2023) use a CRISPR‐based approach to systematically create TS alleles across essential genes in E. coli
Temperature affects virtually all biophysical processes inside cells. Relevantly, it shifts over tim...
Temperature-sensitive (Ts) mutants are a powerful tool with which to study gene function in vivo. Ts...
Recently, the Cas9-CRISPR system has proven to be a useful tool for genome editing in eukaryotes, wh...
Abstract Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular syste...
The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli...
<div><p>Previous screening of a single-gene knockout library consisting of 3,908 disrupted-mutant st...
Organisms have evolved to exploit new environments by processes that involve both mutations and gene...
The heat shock response is a highly conserved genetic mechanism which is induced by a wide range of ...
The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli...
Exploring the phenotypes associated with conditional alleles of essential genes has been widely used...
High-throughput genetic screens are powerful methods to identify genes linked to a given phenotype. ...
International audienceHigh-throughput genetic screens are powerful methods to identify genes linked ...
Bacterial essential genes contribute to the most fundamental processes of cellular life. The study o...
High-throughput genetic screens are powerful methods to identify genes linked to a given phenotype. ...
Temperature can be used to control engineered E. coli — for example, the living component of an engi...
Temperature affects virtually all biophysical processes inside cells. Relevantly, it shifts over tim...
Temperature-sensitive (Ts) mutants are a powerful tool with which to study gene function in vivo. Ts...
Recently, the Cas9-CRISPR system has proven to be a useful tool for genome editing in eukaryotes, wh...
Abstract Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular syste...
The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli...
<div><p>Previous screening of a single-gene knockout library consisting of 3,908 disrupted-mutant st...
Organisms have evolved to exploit new environments by processes that involve both mutations and gene...
The heat shock response is a highly conserved genetic mechanism which is induced by a wide range of ...
The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli...
Exploring the phenotypes associated with conditional alleles of essential genes has been widely used...
High-throughput genetic screens are powerful methods to identify genes linked to a given phenotype. ...
International audienceHigh-throughput genetic screens are powerful methods to identify genes linked ...
Bacterial essential genes contribute to the most fundamental processes of cellular life. The study o...
High-throughput genetic screens are powerful methods to identify genes linked to a given phenotype. ...
Temperature can be used to control engineered E. coli — for example, the living component of an engi...
Temperature affects virtually all biophysical processes inside cells. Relevantly, it shifts over tim...
Temperature-sensitive (Ts) mutants are a powerful tool with which to study gene function in vivo. Ts...
Recently, the Cas9-CRISPR system has proven to be a useful tool for genome editing in eukaryotes, wh...
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