Unveiling the draft genome sequence of diesel-degrading Paenibacillus sp. strain d9, a surfactant producer isolated from diesel-contaminated soil

Introduction: Gram-positive bacteria, particularly Bacillus and Paenibacillus spp., have gained significant attention for their potential in environmental bioremediation (biosurfactant production) and diverse biotechnological applications. Among these, Paenibacillus sp. D9, isolated from oil-contaminated soil, has shown diesel and engine oil degradation capabilities and biosurfactant production. However, its role in alkane degradation remains unexplored. Methodology and Result: To shed light on its unique attributes, we conducted whole-genome sequencing of Paenibacillus sp. D9 using the Illumina HiSeq 2000 platform. The draft genome comprised 56 contigs and 7 scaffolds, with a size of 5,645,302 bp at 157.94× coverage and a G + C content of 58.13%. A total of 9,950 Coding Sequences (CDSs) were predicted, and functional annotation revealed 3,283 (43.19%) and 3,155 (58.8%) putative genes based on Bacterial Annotation System (BASys) and Rapid Annotation using the Subsystems Technology (RAST) subsystem categorization, respectively. Furthermore, 93 tRNA and 23 rRNA genes were identified. Conclusion: This genome announcement provides valuable insights into the genetic potential of Paenibacillus sp. D9 and paves the way for future research in its biotechnological applications.