Developmental potential and mtDNA genetic drift analysis in mouse SCCT-generated embryos in vitro.

(A) Morphological images of derived mouse ESCs in the control, Con-SCCT, and MRR-SCCT groups. Scale bars, 50 μm. (B) MtDNA residual levels in mouse ES cell lines (%, Y-axis) during 21 passages in the control, Con-SCCT, and MRR-SCCT groups (X-axis). The error bars represented SEM with mean values shown in each group. (C) MtDNA mean residue levels in mouse ES cell lines (%, Y-axis) in the control, Con-SCCT, and MRR-SCCT groups (X-axis). The error bars represented SEM with mean values shown in each group. The whiskers indicated a significant difference between groups (**** denoted P p > 0.05, ns: not significant). The numerical data were listed in S5 Data. Con-SCCT, conventional SCCT; mtDNA, mitochondrial DNA; MRR, maximal residue removal; SCCT, spindle-chromosomal complex transfer.