Time course of ZIKV RNA levels in placentas, fetal bodies, and fetal heads during vertical transmission.

Pregnant AIR mice were inoculated at 7 days post-mating. A-D) At 7, 11, 13, and 14 dpi, mice were euthanized and tissues analyzed by RT-qPCR for ZIKV RNA in (A) placentas, (B) whole fetus or fetal bodies, and (C) fetal heads. D) ZIKV RNA levels in fetal bodies selected for sequencing and the matched head (not sequenced) is plotted with lines connecting tissue from the same fetus. Dashed lines denote mock-inoculated levels in the tissues. Each dam is represented by a different color, and each tissue by an individual symbol.