The activity of TCAMS compound identified in the Pf DGFA screen.

(A) 85 molecules were confirmed active in the Pf DGFA. 58 were previously identified in one or more transmission-blocking screens. (B) The majority of molecules displayed similar activity against male and female gametocytes, or biased/specific activity against male gametocytes. No molecule was identified with >4.7-fold greater activity against female gametocytes. Abbreviations (IC50 = concentration giving 50% inhibition, PbODA = P. berghei Ookinete Development Assay [5], PfDGFA = P. falciparum Dual Gamete Formation Assay, PfFGAA = P. falciparum Female Gametocyte Activation Assay [7], PfGCT = P. falciparum Gametocyte Viability ATP Assay [18]).