[Comparative study of two procedures for the gaschromatographic determination of fluoride in physiological fluids.]

Abstract niet beschikbaarThis report describes the results of a comparison of two analytical procedures for the determination of fluoride in physiological fluids: SOP nr. ARO/024* and SOP nr. ARO/073. Both methods are based on the gaschromatographic determination of trimethylfluorosilane (TMFS) resulting from the derivatization of fluoride with trimethylchlorosilane (TMCS) under acid conditions. Materials used for this comparison were: 21 rat plasma samples from an experimental animal study with hydrogenfluoride, a pooled sample of pig serum and an urine reference sample with certified fluoride content from the "Behring Institut" in Germany. Results obtained with both methods for rat plasma and the reference sample do not differ significantly. However, those for the pooled serum do! The average result obtained with SOP nr. ARO 024* for the reference urine is identical with the target reference value for fluoride. Precision of results for SOP nr. ARO/024* is better than for SOP nr. ARO/073. However, this difference in precision is not significant. SOP nr. ARO/073 will be replaced by the procedure described in SOP nr. ARO/024*.RIV