Ceramide Aminoethylphosphonate in the Egg of the JANBOTANISHI, Ampullarius urceus

Ceramide aminoethylphosphonate was isolated from the egg of the JANBOTANISHI, Ampullarius urceus. The compound was purified with a combination of mild alkaline hydrolysis of the total lipids, QAE-Sephadex-A 25 column chromatography and two dimensional thin layer chromatography. the infrared spectrum showed an absorption band at 1180 cm-1 due to c-p bond, and was essentially identical with that of the standard ceramide aminoethylphosphonate. Upon hydrolysis of the tance by strong acid, neither change in the chromatographic behaviors of this substance nor liberation of inorganic phosphate was observed. The stability of the compound to acid hydrolysis suggested the presence of a c-p bond. On comparison with synthetic compound, the aqueous hydrolysis product behaved like 2-aminoethylphosphonic acid on thin layer chromatograms. The Predominant fatty acids were palmitic acid, eicosapentaenoic acid, erucic acid and docosatrienoic acid