Molecular Cloning of the Japanese Quail α A Globin cDNA

Double stranded cDNA was synthesized using the poly (A) + RNA of the Japanese quail reticulocytes and ligated into the EcoRI site ofλgt 10 phage DNA. A recombinant phage,λQαG1 was selected by plaque hybridization using a 30mer synthetic oligonucleotide probe specific to the α globin gene. The size of the cDNA insert in the recombinant phage DNA was 0.53 kb and the restriction map was similar to that of the chicken α A globin gene. Nucleotide sequencing of the cDNA insert indicated that it contained the entire coding information for the α A globin