The Experimental Study on Lung Transplantation

Previous studies in the laboratory have been shown that homografts of the lung in canine can survive extended period of time, if methotrexate is given to the recipient animal, Several homografts survive more than a month. A series of fifty unrelated mongrel pairs have been subjected to, homotransplantation of the left lung. In each case the donor lung was stored in vitro 2 to 12 hours before placement in the recipient animal. The lung was perfusated with 5% dextrose, 3.6% PVP and plasma at 4°C by infusion pump and was ventilated with room air by respirator. It has been eluciated by histologic and electromicroscopic study including autopsy and survival rate that the safety storage period had been within 4 to 6 hours to prevent a fine change on donor lung due to storages