Dengue IgM-capture ELISA Using Heat-inactivated and Unheated Control Antigens Prepared as Infected C6/36 Cell Culture Fluid

A panel of 91 dengue patient's sera were examined in the IgM-capture ELISA using each of the 4 types of dengue antigens prepared as infected C6/36 cell culture fluid, with or without brief heat inactivation at 56℃. The result did not show any significant difference between the results obtained by both antigens. Therefore, dengue antigens for serodiagnosis by the IgM-capture ELISA could be prepared as infected C6/36 cell culture fluid with brief heat-inactivation for biosafety requirement