Features of the condition of the neuroendocrine-immune complex in different constellations of entropies of morpho-functional immune subsystems in rats

Background. We have previously shown that the Entropy (E) of the Thymocytogram (T), Splenocytogram (S) as well as Leukocytogram (L) and Immunocytogram (I) of the blood in rats are virtually independent of each other. The purpose of this study was to clarify the characteristics of the condition of the neuroendocrine-immune complex in different constellations of E(s) of these four morpho-functional immune subsystems. Materials and methods. Experiment was performed on 108 healthy Wistar rats (48 male and 60 female) weighing 240-290 g divided into 8 groups. Animals of the first group remained intact. Instead, the other rats received various balneofactors for 6 days. The day after the completion of the drinking/application course the parameters of neuroendocrine-immune complex were registered. Results. The method of cluster analysis created four homogeneous groups of rats, significantly different from each other in the constellation of E of four morpho-functional immune subsystems. The members of the first cluster (n=31) are characterized by elevated levels of E (Z±SE) of I (1,12±0,16), T (1,00±0,14) and L (0,88±0,12) at the normal E level of S (0,14±0,16). The members of the second cluster (n=21) are characterized by reduced E level of L (-1,59±0,16) in combination with increased levels of I (0,80±0,17) and T (0,81±0,15) at the normal E level of S (0,13±0,16). A characteristic feature of the members of the third cluster (n=28) is reduced E of T (-1,01±0,17) at normal E levels of S (-0,33±0,23), I (-0,19± 0,14 ) and L (0,35±0,20). The last cluster (n=28) is characterized by reduced E of I (-1,36± 0,14), while the E of other immune subsystems are at the borderline levels: L (-0,52±0,16), S (0,49±0,15), T (0,60±0,15). The method of discriminant analysis revealed 15 parameters of the neuroendocrine-immune complex, the set of which four clusters of Entropies differ from each other with an accuracy of 95%. Conclusion. Each constellation of independent Entropies of the thymocytogram, splenocytogram, immunocytogram and leukocytogram is accompanied by a specific constellation of parameters of the neuroendocrine-immune complex which indicates their natural functional relationships