Primary amine is one of the most prevalent moieties in synthetic intermediates and pharmaceutical compounds. The preparation of aliphatic primary amines via C−H functionalization would provide direct access to the nitrogen-containing compounds from hydrocarbon substrates. While the enzymatic oxyfunctionalization of C–H bonds is well established, the analogous strategy for nitrogen incorporation is unknown in Nature. Likewise, a synthetic method for selective primary amination of aliphatic C–H bonds remains elusive. Combining chemical intuition and inspiration from Nature, chemists and protein engineers have created new heme-containing enzymes for the C(sp³)–H primary amination via directed evolution. This thesis describes some of the effort...
Heme proteins, in particular cytochromes P450, have been extensively used in biocatalytic applicatio...
Despite the astonishing diversity of naturally occurring biocatalytic processes, enzymes do not cata...
Heme enzymes have the potential to be widely used as biocatalysts due to their capability to perform...
Primary amine is one of the most prevalent moieties in synthetic intermediates and pharmaceutical co...
One of the most important challenges in chemistry is the creation of new catalysts. Nature excels at...
Hydroxylamine-derived reagents have enabled versatile nitrene transfer reactions for introducing nit...
Engineered heme protein biocatalysts provide an efficient and sustainable approach to develop amine-...
Selective catalytic functionalization of C(sp 3)-H bonds represents a powerful means to access valua...
C-H functionalization is an attractive strategy to construct and diversify molecules. Heme proteins,...
C–H bonds are ubiquitous structural units of organic molecules. Although these bonds are generally c...
We recently demonstrated that variants of cytochrome P450<sub>BM3</sub> (CYP102A1) catalyze the inse...
We recently demonstrated that variants of cytochrome P450_(BM3) (CYP102A1) catalyze the insertion of...
Adapting enzymes for chemical synthesis is a longstanding and contemporary challenge; however, enzym...
Nitrogen activation: Though P450 enzymes are masters of oxygen activation and insertion into C-H bon...
Cytochrome P450 enzymes were recently engineered to catalyze the C–H amination reaction of aryl sulf...
Heme proteins, in particular cytochromes P450, have been extensively used in biocatalytic applicatio...
Despite the astonishing diversity of naturally occurring biocatalytic processes, enzymes do not cata...
Heme enzymes have the potential to be widely used as biocatalysts due to their capability to perform...
Primary amine is one of the most prevalent moieties in synthetic intermediates and pharmaceutical co...
One of the most important challenges in chemistry is the creation of new catalysts. Nature excels at...
Hydroxylamine-derived reagents have enabled versatile nitrene transfer reactions for introducing nit...
Engineered heme protein biocatalysts provide an efficient and sustainable approach to develop amine-...
Selective catalytic functionalization of C(sp 3)-H bonds represents a powerful means to access valua...
C-H functionalization is an attractive strategy to construct and diversify molecules. Heme proteins,...
C–H bonds are ubiquitous structural units of organic molecules. Although these bonds are generally c...
We recently demonstrated that variants of cytochrome P450<sub>BM3</sub> (CYP102A1) catalyze the inse...
We recently demonstrated that variants of cytochrome P450_(BM3) (CYP102A1) catalyze the insertion of...
Adapting enzymes for chemical synthesis is a longstanding and contemporary challenge; however, enzym...
Nitrogen activation: Though P450 enzymes are masters of oxygen activation and insertion into C-H bon...
Cytochrome P450 enzymes were recently engineered to catalyze the C–H amination reaction of aryl sulf...
Heme proteins, in particular cytochromes P450, have been extensively used in biocatalytic applicatio...
Despite the astonishing diversity of naturally occurring biocatalytic processes, enzymes do not cata...
Heme enzymes have the potential to be widely used as biocatalysts due to their capability to perform...